Rapid Commun Mass Spectrom. 2009 Jul 28; 23(17): 2724-2732Wang Y, Ding C, Du K, Xiao Y, Wu C, Zhang J, Qin H, Du GXiao-xu-ming decoction (XXMD) prescription is a traditional Chinese prescription that has been widely used to treat theoplegia and the sequela of theoplegia. Modern pharmacological research has also indicated that the active fraction from XXMD is able to treat cardiovascular diseases and Alzheimer's disease. In the study reported here, high-performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry (HPLC/FTICR-MS) was developed to identify active compounds and their metabolites after oral administration of active fraction from Xiao-xu-ming decoction to rats, using parent mass list triggered data-dependent multiple-stage mass analysis at a resolving power of 100 000 in the external calibration mode. The mass accuracies obtained for full-scan MS were within 2 ppm in most cases. Fifteen constituents were identified in the active fraction from XXMD and the biological samples of rats. The fragmentation behaviors of these constituents were summarized which would be helpful for structural characterization. The profiles of the constituents in the active fraction and biological samples of rats were obtained which provided us with much information for a better understanding of the chemical basis of the pharmacologic actions of XXMD. Copyright (c) 2009 John Wiley & Sons, Ltd.

Angew Chem Int Ed Engl. 2009 Jul 23; Zhao L, Xie F, Cheng G, Hu Y

Biomol NMR Assign. 2009 Jun; 3(1): 95-7Zhang F, Guo C, Lou L, Lin DLipocalin2 plays an important role in the innate immune system. In this article we report the backbone and side-chain resonance assignments of rat lipocalin2 (rLcn2). These assignments provide a basis for determining the structure and dynamics of rLcn2.

Zhongguo Zhong Yao Za Zhi. 2009 Apr; 34(7): 861-3Liu A, Xu L, Zou Z, Yang SOBJECTIVE: To isolate and identify chemical constituents of Cassia alata. METHOD: Compounds were separated and purified by column chromatography with silica gel and Sephadex LH-20, elucidated by spectroscopic methods including 1D and 2D NMR, IR and MS techniques. RESULT: Twelve compounds were isolated from C. alata, which were identified as chrysoeriol (1), kaempferol (2), quercetin (3), 5,7,4'-trihydroflavanone (4), kaempferol-3-O-beta-D-glucopyranoside (5), kaempferol-3-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside (6), 17-hydrotetratriacontane (7), n-dotriacontanol (8), n-triacontanol (9), palmitic acid ceryl ester (10), stearic acid (11), palmitic acid (12). CONCLUSION: Compounds 3-12 were isolated from C. alata for the first time.