ChemMedChem. 2009 May 8; Chen F, Gao AH, Li J, Nan FJ

J Asian Nat Prod Res. 2009; 11(4): 289-93Zhang YW, Sun WX, Li X, Zhao CC, Meng DL, Li NTwo new compounds were isolated from the whole plant of Helichrysum arenarium (L.) Moench. By means of spectroscopic data (IR, UV, 1D and 2D NMR, HR-MS, ESI-MS, and NOESY) and chemical evidence, the structures were established as 6,7-dimethoxy-4-hydroxy-1-naphthoic acid (1) and (Z)-5-hydroxy-7-methoxy-4-[3-methyl-4-(O-beta-D-xylopyranosyl)but-2-enyl]isobenzofuran-1(3H)-one (2).

J Pharm Biomed Anal. 2009 Apr 17; Yang D, Yu C, Li S, Liu G, Lu Y, Wang YA sensitive and specific LC-CMS/MS method was developed for the quantification of changrolin, an anti-arrhythmic drug, in rat plasma using tiapride as internal standard. Liquid-liquid extraction was employed for sample preparation and analyzed using a multiple reaction monitoring mode with electrospray positive ionization source. The calibration curve for changrolin was linear over the range 5-1600ng/mL with coefficients of correlation >0.99. The intra- and inter-batch precision was less than 8.6%, and accuracy ranged from 94.6% to 104.5%. This validated LC-MS/MS method was successfully applied to a bioavailability study of oral and intravenous administration of changrolin with 20mg/kg dosage in SD rats.

Cancer Sci. 2009 Apr; 100(4): 782-5Gao H, Lu Q, Liu X, Cong H, Zhao L, Wang H, Lin D1H nuclear magnetic resonance (NMR)-based metabonomics was used to characterize metabolic profiles of liver cirrhosis (LC) and hepatocellular carcinoma (HCC). We found compared to healthy humans, LC and HCC sera had higher levels of acetate, Nacetylglycoproteins, pyruvate, glutamine, alpha-ketoglutarate, glycerol, tyrosine, 1-methylhistidine and phenylalanine, together with lower levels of low-density lipoprotein, isoleucine, valine, acetoacetate, creatine, choline and unsaturated lipids. Scores plot of pattern recognition analysis were capable of distinguishing LC and HCC patients from healthy humans. These results indicate that serum NMR spectra combined with pattern recognition analysis techniques offer an efficient, convenient way of depicting tumor biochemistry, which may be of great benefit to early diagnosis of human malignant diseases using single blood samples.

Steroids. 2009 May 14; Zhang T, Liu H, Liu XT, Chen XQ, Wang QTwo new steroidal saponins, padelaosides A (1) and B (2), along with two other known steroidal saponins (3 and 4) were isolated from the rhizomes of Paris delavayi. Their structures were elucidated by 1D and 2D NMR techniques, HRFTMS, physical data and chemical methods. The two different absolute configurations of fucose, assigned as L and D, that were found on compounds 1 and 2, respectively, were simultaneously reported in a natural medicine for the first time.