Zhongguo Zhong Yao Za Zhi. 2009 Apr; 34(8): 1005-6Zhang C, Xiao Y, Taniguchi M, Baba KOBJECTIVE: To study the chemical constituents in roots of Peucedanum praeruptorum. METHOD: The constituents were isolated by column chromatography on silica gel and ODS, and identified by spectroscopic methods. RESULT: Seven compounds, alpha-D-glucopyranose-1-hexadecanoate (1), D-mannitol monohexadecanoate (2), adenosine (3), butyric acid (4), eleutheroside B, (5), apiosylskimmin (6), and mannitol (7) were isolated and identified. CONCLUSION: Compounds 1-5 were isolated from this plant for the first time.

Zhongguo Zhong Yao Za Zhi. 2009 Apr; 34(8): 969-72Du M, Liu S, Li M, Jin R, Kang C, Li JOBJECTIVE: To investigate the regularity of Yulian Cataplasm in vitro release and transdermal behaviors. METHOD: Improved Franz diffusion devices was used with four index ingredients as evodiamine, rutaecarpine, palmatine and berberine that were determined by HPLC in one mobile phase. RESULT: The release rates of evodiamine, rutaecarpine, palmatine and berberine were 0.0239, 0.0156, 0.0725, 0.8191 mg x cm(-2) x h(-1/2), respectivley. The transdermal rates of evodiamine, rutaecarpine, palmatine and berberine were 1.256, 1.0302, 2.8029, 20.919 microg x cm(-2) x h(-1), respectively. CONCLUSION: The releasing process of all index is in accordance with Higuchi equation and the transdermal proccess is in accordance with zero-level equation.

Rapid Commun Mass Spectrom. 2009 Jul 28; 23(17): 2724-2732Wang Y, Ding C, Du K, Xiao Y, Wu C, Zhang J, Qin H, Du GXiao-xu-ming decoction (XXMD) prescription is a traditional Chinese prescription that has been widely used to treat theoplegia and the sequela of theoplegia. Modern pharmacological research has also indicated that the active fraction from XXMD is able to treat cardiovascular diseases and Alzheimer's disease. In the study reported here, high-performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry (HPLC/FTICR-MS) was developed to identify active compounds and their metabolites after oral administration of active fraction from Xiao-xu-ming decoction to rats, using parent mass list triggered data-dependent multiple-stage mass analysis at a resolving power of 100 000 in the external calibration mode. The mass accuracies obtained for full-scan MS were within 2 ppm in most cases. Fifteen constituents were identified in the active fraction from XXMD and the biological samples of rats. The fragmentation behaviors of these constituents were summarized which would be helpful for structural characterization. The profiles of the constituents in the active fraction and biological samples of rats were obtained which provided us with much information for a better understanding of the chemical basis of the pharmacologic actions of XXMD. Copyright (c) 2009 John Wiley & Sons, Ltd.

Rapid Commun Mass Spectrom. 2009 Jul 28; 23(17): 2724-2732Wang Y, Ding C, Du K, Xiao Y, Wu C, Zhang J, Qin H, Du GXiao-xu-ming decoction (XXMD) prescription is a traditional Chinese prescription that has been widely used to treat theoplegia and the sequela of theoplegia. Modern pharmacological research has also indicated that the active fraction from XXMD is able to treat cardiovascular diseases and Alzheimer's disease. In the study reported here, high-performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry (HPLC/FTICR-MS) was developed to identify active compounds and their metabolites after oral administration of active fraction from Xiao-xu-ming decoction to rats, using parent mass list triggered data-dependent multiple-stage mass analysis at a resolving power of 100 000 in the external calibration mode. The mass accuracies obtained for full-scan MS were within 2 ppm in most cases. Fifteen constituents were identified in the active fraction from XXMD and the biological samples of rats. The fragmentation behaviors of these constituents were summarized which would be helpful for structural characterization. The profiles of the constituents in the active fraction and biological samples of rats were obtained which provided us with much information for a better understanding of the chemical basis of the pharmacologic actions of XXMD. Copyright (c) 2009 John Wiley & Sons, Ltd.

Zhongguo Zhong Yao Za Zhi. 2009 Apr; 34(8): 1015-7Xiong Y, Gao H, Wang Z, Zhu JOBJECTIVE: To investigate ingredient differences of Lonicera japonica bud processed by different dryness techniques. METHOD: The HPLC fingerprint was used to evaluate the quality of L. japonica by various dryness process. The analysis was performed on a Kromasil C18 (4.6 mm x 250 mm, 5 microm). Acetonitril and water containing 0.4% H3PO4 were used as mobile phases with gradient elution. The flow rate was set at 1.0 mL x min(-1), the column temperature was set at 30 degrees C, and the detection wavelength was at 238 nm. RESULT: The HPLC-fingerprint profiles of L. japonica from six different dryness techniques were determined. A total of 17 common peaks were found, but their contents from various dryness techniques were significantly different. CONCLUSION: HPLC-fingerprint technique was one of potential Process Analytical Techniques (PAT) to explore the ingredient differences in dryness process.