Proteomic analysis of possible target-related proteins of cyclophosphamide in mice thymus.

Food Chem Toxicol. 2009 May 4; Ma C, Yue QX, Guan SH, Wu WY, Yang M, Jiang BH, Liu X, Guo DACyclophosphamide (Cy) could induce immuno-suppression such as thymus atrophy and inhibition of lymphocyte proliferation response in mice. But;the mechanism of its effect is still not clear. Polysaccharides isolated from spore of Garnoderma lucidum (GL-SP) could;at least partly;restore the immunological effects against Cy-induced immuno-suppression. In the present study;a two-dimensional gel electrophoresis (2-DE)-based comparative proteomic method was conducted to identify the possible target-related proteins of Cy in mice thymus including those could be influenced by GL-SP. Fifteen proteins differentially expressed in Cy-treated mice compared with control were identified. Among the fifteen proteins;combined use of GL-SP with Cy could not affect Cy-induced expression change of three proteins;totally prevented Cy-induced expression change of six proteins;partly prevented Cy-induced expression change of four proteins and further enhanced Cy-induced expression change of two proteins. Results of the present study shed light on the mechanism of Cy-induced immuno-suppression as well as the protective effect of GL-SP against toxicity of Cy.